To find the DNA they needed, scientists cut a round hole in the stone church floor above the family crypt of Florentine silk merchant Francesco del Giocondo. The tomb lies behind the altar of the Santissima Annunziata Basilica.
Writer and researcher Silvano Vinceti plans to compare DNA from the bones with that of three women buried at the nearby convent of Saint Ursula.
Lisa Gherardini died there as a nun in 1542.
It is hoped that some of the bones will belong to at least one of her blood relation, probably her son, Piero.
"When we find a match between mother and child - then we will have found the Mona Lisa," said Mr Vinceti.
He added that once a DNA match is made, an image of Gherardini's face can be generated from the skull and compared with the painting.
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